UH 세포의 세포사멸이 조직세포의 증식과 이동에 미치는 영향
Efects of Osteocyte Apoptosis on Bone Cel Proliferation and Recruitment
이희수, 고성희,
소속 상세정보
이희수 ( Lee Hee-Su ) - 강릉원주대학교 치과대학 구강해부학교실
고성희 ( Ko Sung-Hee ) - 강릉원주대학교 치과대학 약리학교실
KMID : 0355820140350010023
Abstract
Osteocytes are the most abundant cels in bone and communicate with other bone surface cels like osteoblasts, osteoclasts, osteoclast precursors and bone lining cels through their networking in canaliculi. Osteocyte apoptosis by microcracks has ben suggested the leading step in targeted bone remodeling. Therefore, to determine the role for osteocyte apoptosis in bone remodeling, we examined the efect of apoptotic osteocytes on osteoclast precursors and osteoblasts proliferation and recruitment. We used the MLO-Y4 cels as in vitro model for osteocytes, RAW264.7 cels as osteoclast precursors and 2T3 cels as osteoblasts. For induction of MLO-Y4 cel apoptosis, MLO-Y4 cels were treated with 100 μM etoposide for 6 hours and apoptosis was determined with increase of casapase-3 activity and ratio of trypan
blue straining cels. MLO-Y4 cels conditioned medium (Y4-CM) was colected for 24h culture after apoptosis induction.
For proliferation asay, RAW 264.7 cels and 2T3 cels were cultured with 0, 20, 50% healthy or apoptotic Y4 CM. Cultures were stopped after 3 days and cel number was counted. The migration of RAW 264.7 and 2T3 cels was asayed using transwels with healthy or apoptotic 20, 50% Y4 -CM. Healthy Y4-CM increased osteoclast precursor proliferation and migration. Healthy Y4-CM decreased 2T3 cel proliferation and migration. However apoptotic Y4-CM induced by etoposide increased the proliferation of 2T3 cels compared to control (healthy) Y4-CM. Apoptotic Y4 CM increased RAW 264.7 cell proliferation and had no effect on migration of RAW 264.7 cells and 2T3 cells. These results suggest that osteocyte apoptosis may play a role in bone remodeling by changes in signals linked to other bone cells.
키워드
Osteocyte apoptosis; Proliferation of osteoblast and osteoclast precursors; Migration of osteoblast and osteoclast precursors
원문 및 링크아웃 정보
등재저널 정보